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Bioss nfdm
Nfdm, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrin+alpha+v+++beta1+antibody/Integrin+Alpha+V+%2B+Beta1+Antibody/pm42092083-439-155-151
Average 94 stars, based on 14 article reviews

nfdm - by Bioz Stars, 2026-10

94/100 stars

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Blocking Assay:

Article Title: Targeting the D93 cryptic collagen epitope alters integrin α2β1-dependent cellular migration and collagen remodeling in metastatic breast cancer.
Article Snippet: .. Target Manufacturer & Product Number Host Species of Primary Antibody Blocking Solution (in 1X PBS) Primary Antibody (in 1X PBS) Secondary Antibody (in 1X PBS) Cytation 5 Exposure Settings D93 Tracom Pharmaceuticals, NA Humanized Mouse NA 20 μg/mL in 2.5% BSA 1:100 in 2.5% BSA NA Integrin α1β1 Invitrogen, MA5-35041 Mouse 5% NFDM 1:1000 in 5% BSA 1:400 in 5% BSA LED Int: 10 Int Time: 1100 Cam Gain: 24 Integrin α2β1 Invitrogen, MA1-26714 Mouse 5% NFDM 1:500 in 5% BSA 1:400 in 5% BSA LED Int: 10 Int Time: 350 Cam Gain: 24 AR TIC LE IN PR ES S Integrin α10β1 Invitrogen, PA5-100840 Rabbit 5% NFDM 1:200 in 5% BSA 1:400 in 5% BSA LED Int: 10 Int Time: 81 Cam Gain: 24 Integrin α11β1 Invitrogen, MA5-24758 Mouse 5% NFDM 1:250 in 5% BSA 1:400 in 5% BSA LED Int: 10 Int Time: 164 Cam Gain: 24 Integrin αVβ1 Bioss, BS-2016R Rabbit 5% NFDM 1:200 in 5% BSA 1:400 in 5% BSA LED Int: 10 Int Time: 27 Cam Gain: 24 DAPI Invitrogen, D1306 NA NA NA 1:1000 LED Int: 10 Int Time: 5 Cam Gain: 10 AR TIC LE IN PR ES S ..

Article Title: Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repair
Article Snippet: .. Cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions, followed by incubation of the primary antibody (1 μg/10 6 cells, bs-2016R, Bioss, China; ab275977, Abcam, UK; ab30394, Abcam, UK) for 30 min at room temperature. .. The IgG isotype control antibody (ab170190, Abcam, UK) was used at the same concentration and conditions as the primary antibody.

Article Title: Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repair.
Article Snippet: .. Cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions, followed by incubation of the primary antibody (1 μg/106 cells, bs-2016R, Bioss, China; ab275977, Abcam, UK; ab30394, Abcam, UK) for 30 min at room temperature. .. The IgG isotype control antibody (ab170190, Abcam, UK) was used at the same concentration and conditions as the primary antibody.

other:

Article Title: Macrophage-induced integrin signaling promotes Schlemm's canal formation to prevent intraocular hypertension and glaucomatous optic neuropathy.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-Armenian Hamster IgG Alexa Fluor 488 (1:800) Jackson ImmunoResearch Cat# 127-545-160; BRID: AB_2338997 Goat anti-Armenian Hamster IgG Cyanine Cy3 (1:800) Jackson ImmunoResearch Cat# 127-165-160; BRID: AB_2338989 Goat anti-Armenian Hamster IgG Alexa Fluor 647 (1:800) Jackson ImmunoResearch Cat# 127-605-160; BRID: AB_2339001 Goat anti-Mouse IgG HRP (1:2000) Jackson ImmunoResearch Cat# 115-035-003; BRID: AB_10015289 Goat anti-Rabbit IgG HRP (1:2000) Jackson ImmunoResearch Cat# 111-035-003; BRID: AB_2313567 Goat anti-Rat IgG HRP (1:2000) Proteintech Cat# SA00001-15; BRID: AB_2864369 Donkey anti-Goat lgG HRP (1:2000) Proteintech Cat# SA00001-3; BRID: AB_2890882 Chemicals, peptides, and recombinant proteins AKT activator SC79 Millipore 123871 DNase I Sigma DN25-100MG EdU Thermo Fisher C10337 Endothelial basal media (EBM2) LONZA CC-3156 EGM-2 SingleQuotsTM Supplements LONZA CC-4147 Fibronectin Sigma F2006 FOXO1 inhibitor AS1842856 Calbiochem 344355 Laemmli buffer BIO-RAD 1610737 Phosphatase inhibitor cocktail Roche 04906837001 Proteinase inhibitor cocktail Roche 11697498001 siControl (siCTRL) QIAGEN 1027281 Tamoxifen Sigma T5648 Toluidine blue Servicebio G1032 Tropicamide ophthalmic solution SINQI H20055546 Type I collagenase Worthington LS004196 Type IV collagenase Worthington LS004188 Vitronectin Biolegend 757708 Critical commercial assays BCA Protein Assay Kit Thermo Fisher 23227 RNeasy Plus Mini Kit QIAGEN 74134 RNA sequencing Gene De-novo Biotechnology Co. (Guangzhou, China) N/A.

Incubation:

Protein-Protein interactions:

Recombinant:

Article Title: Semaphorin 7A is protective during inflammatory peritonitis through integrin receptor signaling.
Article Snippet: After incubation for 4 hours, 100μl supernatant was harvested and analyzed to determine IL-10 following the manufacturer’s instructions via ELISA (R&D Systems, Cat. Nr. DY217B). .. In a subset of experiments, CaCo-2 and MM6 cells were preincubated for 1 hour at room temperature with one of the following antibodies: 1:500 ITGA1 (Anti-Integrin alpha 1 antibody, (Abcam, Cat. Nr. ab33410)) or 1:1000 ITGAV/ITGB1 (Rabbit Anti-Integrin Alpha V + Beta1 Polyclonal Antibody (Bioss, Cat. Nr. bs-2016R)), 1:1000 ITGB1 (Anti-Integrin b1; abcam, Cat Nr. ab52971) or 1:500 Anti-PLXNC1 (Santa Cruz, Cat. Nr. Sc10149), respectively, before adding ZyA and/or recombinant human Semaphorin 7A Fc Chimera Protein. ..

Review